CovidV1, Main, Exploration, bibRecord, 000E81

Persistent memory CD4+ and CD8+ T-cell responses in recovered severe acute respiratory syndrome (SARS) patients to SARS coronavirus M antigen

Identifieur interne : 000E81 ( Main/Exploration ); précédent : 000E80; suivant : 000E82

Persistent memory CD4+ and CD8+ T-cell responses in recovered severe acute respiratory syndrome (SARS) patients to SARS coronavirus M antigen

Auteurs : Litao Yang [République populaire de Chine] ; Hui Peng [République populaire de Chine] ; Zhaoling Zhu [République populaire de Chine] ; Gang Li [République populaire de Chine] ; Zitong Huang [République populaire de Chine] ; Zhixin Zhao [République populaire de Chine] ; Richard A. Koup [États-Unis] ; Robert T. Bailer [États-Unis] ; Changyou Wu [République populaire de Chine]

Source :

The Journal of general virology [ 0022-1317 ] ; 2007.

RBID : PMC:2362397

Abstract

The membrane (M) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) is a major glycoprotein with multiple biological functions. In this study, we found that memory T cells against M protein were persistent in recovered SARS patients by detecting gamma interferon (IFN-γ) production using ELISA and ELISpot assays. Flow cytometric analysis showed that both CD4⁺ and CD8⁺ T cells were involved in cellular responses to SARS-CoV M antigen. Furthermore, memory CD8⁺ T cells displayed an effector memory cell phenotype expressing CD45RO⁻ CCR7⁻ CD62L⁻. In contrast, the majority of IFN-γ⁺ CD4⁺ T cells were central memory cells with the expression of CD45RO⁺ CCR7⁺ CD62L⁻. The epitope screening from 30 synthetic overlapping peptides that cover the entire SARS-CoV M protein identified four human T-cell immunodominant peptides, p21−44, p65−91, p117−140 and p200−220. All four immunodominant peptides could elicit cellular immunity with a predominance of CD8⁺ T-cell response. This data may have important implication for developing SARS vaccines.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362397

DOI: 10.1099/vir.0.82839-0
PubMed: 17872527
PubMed Central: 2362397

Affiliations:

Le document en format XML

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 T-cell responses in recovered severe acute respiratory syndrome (SARS) patients to SARS coronavirus M antigen</title>
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 T-cell responses in recovered severe acute respiratory syndrome (SARS) patients to SARS coronavirus M antigen</title>
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<wicri:regionArea>Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630</wicri:regionArea>
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<author><name sortKey="Huang, Zitong" sort="Huang, Zitong" uniqKey="Huang Z" first="Zitong" last="Huang">Zitong Huang</name>
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<author><name sortKey="Zhao, Zhixin" sort="Zhao, Zhixin" uniqKey="Zhao Z" first="Zhixin" last="Zhao">Zhixin Zhao</name>
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<author><name sortKey="Koup, Richard A" sort="Koup, Richard A" uniqKey="Koup R" first="Richard A." last="Koup">Richard A. Koup</name>
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<author><name sortKey="Wu, Changyou" sort="Wu, Changyou" uniqKey="Wu C" first="Changyou" last="Wu">Changyou Wu</name>
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<series><title level="j">The Journal of general virology</title>
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<front><div type="abstract" xml:lang="en"><p id="P1">The membrane (M) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) is a major glycoprotein with multiple biological functions. In this study, we found that memory T cells against M protein were persistent in recovered SARS patients by detecting gamma interferon (IFN-<italic>γ</italic>
) production using ELISA and ELISpot assays. Flow cytometric analysis showed that both CD4<sup>+</sup>
 and CD8<sup>+</sup>
 T cells were involved in cellular responses to SARS-CoV M antigen. Furthermore, memory CD8<sup>+</sup>
 T cells displayed an effector memory cell phenotype expressing CD45RO<sup>−</sup>
 CCR7<sup>−</sup>
 CD62L<sup>−</sup>
. In contrast, the majority of IFN-<italic>γ</italic>
<sup>+</sup>
 CD4<sup>+</sup>
 T cells were central memory cells with the expression of CD45RO<sup>+</sup>
 CCR7<sup>+</sup>
 CD62L<sup>−</sup>
. The epitope screening from 30 synthetic overlapping peptides that cover the entire SARS-CoV M protein identified four human T-cell immunodominant peptides, p21−44, p65−91, p117−140 and p200−220. All four immunodominant peptides could elicit cellular immunity with a predominance of CD8<sup>+</sup>
 T-cell response. This data may have important implication for developing SARS vaccines.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
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<name sortKey="Zhao, Zhixin" sort="Zhao, Zhixin" uniqKey="Zhao Z" first="Zhixin" last="Zhao">Zhixin Zhao</name>
<name sortKey="Zhu, Zhaoling" sort="Zhu, Zhaoling" uniqKey="Zhu Z" first="Zhaoling" last="Zhu">Zhaoling Zhu</name>
</country>
<country name="États-Unis"><noRegion><name sortKey="Koup, Richard A" sort="Koup, Richard A" uniqKey="Koup R" first="Richard A." last="Koup">Richard A. Koup</name>
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Persistent memory CD4+ and CD8+ T-cell responses in recovered severe acute respiratory syndrome (SARS) patients to SARS coronavirus M antigen

Persistent memory CD4+ and CD8+ T-cell responses in recovered severe acute respiratory syndrome (SARS) patients to SARS coronavirus M antigen

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Abstract

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